Reciprocal interaction between intestinal microbiota and mucosal lymphocyte in cynomolgus monkeys after alemtuzumab treatmen
发表时间：2013-04-17 15:20
It has been known that the gut microbiota plays a cen- tral role in shaping normal mucosal immunity, how- ever, little information is available whether the variabil- ity of mucosal lymphocytes impacts the commensal flora. Here, we applied a cynomolgus monkey model to characterize the structure and composition of the gut microbiota in response to lymphocyte depletion andtodeterminetheirpotentialassociation.Molecular profilingof 16SrDNAshowedthattheintestinalmicro- biota composition was perturbed after the depletion of mucosal lymphocytes and were recovered follow- ing the repopulation. Some specific bacteria from the orders Lactobacillales, Enterobacteriales and Clostridi- ales, and the genus Prevotella and Faecalibacterium, were primarily responsible for the variations of the gut microbiota after lymphocyte depletion. Interestingly, the species richness of the ileal mucosal microbiota was associated the proportions of TCRa b + or TCRcd + T cells (p < 0.01). We demonstrate for the first time the feature of intestinal microbiota composition after lymphocyte depletion and provide novel evidence that the perturbation of gut microbiota is associated with lymphocyte depletion. It may contribute to understand the relationship between gut commensal microbiota and mucosal immune system. Study results provide insight into biological activity of alemtuzumab in in- testinal barrier in organ transplantation. Key words: Alemtuzumab, denaturing gradient gel electrophoresis, DNA sequencing, intestinal micro- biota, lymphocyte depletion
第一署名医院：南京军区南京总医院
